Two Bioassay Methods to Evaluate Root-Accelerating Activity of Streptomyces sp. MBR52 Metabolites
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- Hasegawa Sachiko
- Institute for Biological Process Research, Akatsuka Garden Co. Ltd.
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- Meguro Akane
- Institute for Biological Process Research, Akatsuka Garden Co. Ltd.
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- Shimizu Masafumi
- Graduate School of Bioresources, Mie University
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- Nishimura Tomio
- Institute for Biological Process Research, Akatsuka Garden Co. Ltd.
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- Toyoda Kazuhiro
- Graduate School of Natural Science and Technology, Okayama University
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- Shiraishi Tomonori
- Graduate School of Natural Science and Technology, Okayama University
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- Kunoh Hitoshi
- Institute for Biological Process Research, Akatsuka Garden Co. Ltd.
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Abstract
Two bioassay methods were established to detect root-growth accelerator(s) among metabolites of Streptomyces sp. strain MBR52. Biological activity of the water-soluble diffusate released from the mycelial mat was tested using broccoli, cucumber, and soybean seedlings. We evaluated root-growth accelerating activity of mat diffusates on adventitious roots emerging from stems of test plants. Among the plants tested, broccoli was most sensitive to the metabolites. The mat diffusate also accelerated growth of its seminal roots. Adventitious and the seminal roots responded differently to auxins. Adventitious root growth increased in response to auxins, whereas seminal root growth did not. The dry weight of adventitious roots increased in response to treatment with an aqueous solution of 0.05 mg/ml freeze-dried metabolites, while elongation of seminal roots responded only to 0.005 mg/ml. The assay methods focusing on both roots reveal different aspects of root-growth accelerating activity of the MBR52 metabolites.
Journal
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- Actinomycetologica
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Actinomycetologica 22 (2), 42-45, 2008
The Society for Actinomycetes Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204702754944
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- NII Article ID
- 10024656861
- 130004487569
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- NII Book ID
- AN10080091
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- ISSN
- 18816371
- 09145818
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- NDL BIB ID
- 9781065
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed