Superoide Dismutase-like Activity of Boar Semen and Effect of SOD on the Preservation of Boar Spermatozoa.

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  • ブタ精しょう中のSOD様活性および保存液中へのSODの添加が精子の運動性に及ぼす影響
  • ブタ精漿中のSOD様活性および保存液中へのSODの添加が精子の運動性に及ぼす影響
  • ブタセイショウチュウ ノ SODヨウ カッセイ オヨビ ホゾンエキ チュウ エ ノ SOD ノ テンカ ガ セイシ ノ ウンドウセイ ニ オヨボス エイキョウ

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The purpose of this experiment was to evaluate the superoxide dismutase (SOD)-like activity of boar seminal plasma, the change of SOD-like activity in the semen stored in Modena extender during preservation and to clarify the effect of addition of SOD to Modena extender on the motility of boar spermatozoa. The SOD-like activity was measured by Electron Spin Resonance Spectroscopy. The SOD-like activities of seminal plasma of boar A and B were 9.7±0.9μ/ml and 6.1±0.2μ/ml, respectively. The SOD-like activity in the semen diluted with Modena extender did not change during the storage period of 28 days at 10°C. The addition of SOD to the extended semen improved sperm viability during storage of 28 days and the best result was obtained from the spermatozoa suspended in Modena extender added 800μ/ml of SOD. The results suggest that the addition of SOD to Modena extender is useful for long-term maintenance of good motility in liquid storage of boar spermatozoa.

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