Persistence of Virus and Antibodies in the Sera of Pigs Experimentally Infected with the Porcine Reproductive and Respiratory Syndrome Virus

SHIBATA Isao, YAZAWA Shigeto, MORI Masafumi

doi:10.12935/jvma1951.54.663

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Other Title

豚繁殖・呼吸障害症候群ウイルス実験感染豚におけるウイルスおよび抗体の長期推移
ブタハンショクコキュウショウガイショウコウグンウイルスジッケンカンセンブタニオケルウイルスオヨビコウタイノチョウキスイイ

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Abstract

Persistence of the humoral immune response against the porcine reproductive and respiratory syndrome virus (PRRSV) was examined by means of the indirect fluorescent antibody (IFA) test, enzyme-linked immunosorbent assay (ELISA), and virus-neutralization (VN) test over a 30-week period in the pigs experimentally infected with the PRRSV. In the first post-inoculation week (PIW), antibodies against the PRRSV were detected by means of IFA tests with anti-swine IgM conjugate (IgM-IFA). They were detected with protein-A conjugate (Protein A-IFA) tests and ELISA during the first and second PIW. They were detected by VN tests from the eighth through the tenth PIW. Although antibody levels gradually declined after reaching maximum values, Protein A-IFA and ELISA continued to detect antibodies throughout the observation period. No antibodies were detected by IgM-IFA after PIW 6 7. According to VN tests, antibody titer, which varied with the individual, remained comparatively low throughout the examination. Viruses were isolated from the sera of inoculated pigs from the 2nd to the 42nd post-inoculation day and from pigs cohabiting with them from the 7th to the 49th post-inoculation day.

Journal

Journal of the Japan Veterinary Medical Association

Journal of the Japan Veterinary Medical Association 54 (9), 663-666, 2001

Japan Veterinary Medical Association

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