Isolation of Peripheral Lymphocytes from New-born Calves by Density Gradient Centrifugation and Lymphocytic Response to Mitogen

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  • 新生子牛の末梢血リンパ球の分離とその幼若化反応
  • シンセイ コウシ ノ マッショウケツ リンパキュウ ノ ブンリ ト ソノ ヨウ

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Abstract

The densities of the separating fluid (Ficoll-Conray) of 1.073-1.077 g/ml were suitable for isolation of lymphocytes from the blood of neonatal calves. The mitogen-induced blastogenic response of lymphocytes isolated from 7 newborn calves was satisfactory when mea suredby DNA fluorometric assay.

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