Serodiagnosis of Fascioliasis by Double Immunodiffusion Test with Blood-Impregnated Filter Paper

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  • SHIMIZU AKIRA
    Department of Animal Hygiene, Faculty of Agriculture, Kobe University
  • AKASHI MIE
    Department of Animal Hygiene, Faculty of Agriculture, Kobe University
  • KAWANO JUNICHI
    Department of Animal Hygiene, Faculty of Agriculture, Kobe University
  • ISHIMARU TSUKASA
    Department of Animal Hygiene, Faculty of Agriculture, Kobe University
  • HAZUE SHINJI
    Department of Animal Hygiene, Faculty of Agriculture, Kobe University
  • KIMURA SHIGE
    Department of Animal Hygiene, Faculty of Agriculture, Kobe University

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Other Title
  • 血液吸着濾紙を用いたゲル内沈降反応による肝蛭症の診断
  • ケツエキ キュウチャク ロシ オ モチイタ ゲルナイ チンコウ ハンノウ ニ

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Abstract

Adult flukes of Japanese Fasciola sp. were washed 4 times with sterile, phosphate-buffered saline (PBS), pH 7.4, and then frozen at -20°C until use. After thawing, one adult fluke was placed in 10% of its weight (10% w/v) of PBS and homogenized. After incubation in a water bath at 37°C for 4 hours, the homogenate was centrifuged at 2, 500 rpm for 10 minutes. The supernatant was used as precipitating antigen for the double immunodiffusion test to make a diagnosis of fascioliasis.<BR>Small discs (5×5 mm) of filter paper impregnated with blood from rabbits and goats infected experimentally with F. hepatica and Japanese Fasciola sp. were allowed to react with the antigen on an agar-gel plate. At least 2 precipitation lines were observed on the plate. When small discs of filter paper impregnated with blood from uninfected rabbits and goats formed no precipitation lines.

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