Detection of Cryptosporidium parvum Pig Genotype Ⅱ and Cryptosporidium suis from Growing-Fattening Pigs Infected with Porcine Circovirus Type 2, and Porcine Reproductive and Respiratory Syndrome Virus in Growing-Fattening Pigs

YUI Takeshi, SHIBAHARA Tomoyuki, KOHMOTO Mariko, YAMASHINA Hisaro, YOSHIDA Terumi, FUKUDA Masaharu, WATANABE Yoshimasa, KUBO Masanori

doi:10.12935/jvma.63.61

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豚サーコウイルス2型および豚繁殖・呼吸障害症候群ウイルスに感染した肥育豚からのCryptosporidium parvum pig genotypeⅡとCryptosporidium suisの検出
豚サーコウイルス2型および豚繁殖・呼吸障害症候群ウイルスに感染した肥育豚からのCryptosporidium parvum pig genotype2とCryptosporidium suisの検出
ブタサーコウイルス 2ガタオヨビブタハンショクコキュウショウガイショウコウグンウイルスニカンセンシタヒイクブタカラノ Cryptosporidium parvum pig genotype2 ト Cryptosporidium suis ノケンシュツ

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Abstract

Half of the growing-fattening pigs on a pig farm in Saitama Prefecture, Japan had exhibited diarrhea and growth retardation since September 2006. In January 2007, four 60-day-old, underdeveloped pigs from the farm were examined. Similar pathological findings were obtained among these pigs. In the lymph nodes, lympho cyte depletion was observed together with infiltration of macrophages, some of which contained cytoplasmic inclusion bodies. Porcine circovirus type 2 (PCV2) antigen was demonstrated in the cytoplasm of macrophages in the lymph nodes. Porcine reproductive and respiratory syndrome virus (PRRSV) was isolated from the lungs of the four pigs. In two of the four pigs, numerous round-to-ovoid organisms were found on the epithelium from the ileum to the colon, histologically. Immunohistochemically, Cryptosporidium parvum oocyst antigen corresponded to the organisms in the intestines. In 30 of 40 (75%) fecal samples of penmates, Cryptosporidium oocysts were detected by immunofluorescent microscopy. Using a polymerase chain reaction restriction fragment length polymorphism technique at the 18S small-subunit ribosomal RNA gene, they were identified as C. parvum pig genotype Ⅱ or C. suis. These findings indicated that Cryptosporidium would be associated with diarrhea in the immunocompromised pigs with PCV2 infection and PRRS.

Journal

Journal of the Japan Veterinary Medical Association

Journal of the Japan Veterinary Medical Association 63 (1), 61-68, 2010

Japan Veterinary Medical Association

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Detection of <i>Cryptosporidium parvum </i>Pig Genotype Ⅱ and <i>Cryptosporidium suis </i>from Growing-Fattening Pigs Infected with Porcine Circovirus Type 2, and Porcine Reproductive and Respiratory Syndrome Virus in Growing-Fattening Pigs