Detection of haploid nuclear death in living Paramecium caudatum

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  • Yang Xianyu
    School of Forestry and Biotechnology, Zhejiang Forestry University
  • Gao Xin
    School of Forestry and Biotechnology, Zhejiang Forestry University
  • Shi Xinlu
    Hangzhou Key Laboratory for Animal Science and Technology, Hangzhou Normal University

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  • Detection of haploid nuclear death in living <i>Paramecium caudatum</i>

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<p>During conjugation of Paramecium caudatum, four haploid nuclei are formed after meiosis, only one of those that enters the paroral region survives and divides once more to form two gametic nuclei, and the remaining three degenerate. Here, we applied the method of co-staining the living cells with acridine orange and Hoechst 33342, which had been found effective to identify the apoptotic nuclei in Tetrahymena, to clarify whether the meiotic nuclear degeneration in P. caudatum is an apoptotic process. We found that three haploid nuclei outside the paroral region were stained green-yellow, while the survivor in the paroral region was stained blue. When the conjugating pairs separated and the synkaryon divided twice, three degenerating haploid nuclei were still observed and stained yellow. The old fragmented macronuclei, which are known to be still alive in exconjugants, were stained blue, in sharp contrast with the case in Tetrahymena. These results strongly suggest that the haploid nuclei degenerate in an apoptotic way in P. caudatum.</p>

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