Development and Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for Quick Identification of Three Japanese Toads
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- ETO Koshiro
- Graduate School of Human and Environmental Studies, Kyoto University
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- MATSUI Masafumi
- Graduate School of Human and Environmental Studies, Kyoto University
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- NISHIKAWA Kanto
- Graduate School of Human and Environmental Studies, Kyoto University
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- HARAMURA Takashi
- Field Science Education and Research Center, Kyoto University
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Description
Three species/subspecies of toads (Bufo japonicus japonicus, B. j. formosus, and B. torrenticola) show parapatric/sympatric patterns of distribution in Japan, and usually they are morphologically indistinguishable at early developmental stages. For quick and easy genetic identification of these three toads, we validated LAMP assay. We designed taxon-specific primers for LAMP assay on mitochondrial cytochrome b region, and these primer sets accurately assigned DNA samples derived from three toads to each taxon. Due to its instancy and simplicity, LAMP method could be a useful diagnostic tool to distinguish taxa which resemble each other.
Journal
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- Current Herpetology
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Current Herpetology 35 (1), 33-37, 2016
The Herpetological Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204712837760
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- NII Article ID
- 130005128163
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- NII Book ID
- AA12410796
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- ISSN
- 18811019
- 13455834
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- HANDLE
- 2433/216862
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- NDL BIB ID
- 027120810
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed