A rapid and convenient method for molecular identification of species of <i>Ammodytes </i>(Perciformes: Ammodytidae) from Japan

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  • Kai Yoshiaki
    Maizuru Fisheries Research Station, Field Science Education and Research Center, Kyoto University
  • Misaka Tadashi
    Wakkanai Fisheries Research Station, Hokkaido Research Organization

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  • 日本産イカナゴ属魚類の簡便な遺伝的識別方法の開発
  • ニホンサン イカナゴゾクギョルイ ノ カンベン ナ イデンテキ シキベツ ホウホウ ノ カイハツ

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<p>A recent taxonomic review has revealed that three species of Ammodytes (Ammodytes japonicus, Ammodytes heian, and Ammodytes hexapterus) are distributed in Japanese waters. Species identification using morphological characters is difficult due to their similar appearance. A rapid and convenient method for identifying the three species of Ammodytes from Japan was developed via a multiplex haplotype-specific (MHS)-PCR of the mitochondrial COI region. Two primers designed for A. japonicus and A. hexapterus yielded species-specific amplification products in different sizes (ca. 500 bp and 300 bp, respectively). Together with a pair of universal primers for fish, about 1,500 bp can be amplified by MHS-PCR, which allows the robust identification of the three species of Ammodytes.</p>

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