An analysis of the biofilms adhered to framework alloys using in vitro denture plaque models

  • URUSHIBARA Yu
    Department of Removable Prosthodontics, Tsurumi University School of Dental Medicine
  • OHSHIMA Tomoko
    Department of Oral Microbiology, Tsurumi University School of Dental Medicine
  • SATO Maki
    Department of Removable Prosthodontics, Tsurumi University School of Dental Medicine
  • HAYASHI Yoshiko
    R & D Department, Sunstar Inc.
  • HAYAKAWA Tohru
    Department of Dental Engineering, Tsurumi University School of Dental Medicine
  • MAEDA Nobuko
    Department of Oral Microbiology, Tsurumi University School of Dental Medicine
  • OHKUBO Chikahiro
    Department of Removable Prosthodontics, Tsurumi University School of Dental Medicine

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タイトル別名
  • An analysis of the biofilms adhered to framework alloys using <i>in vitro</i> denture plaque models
  • An analysis of the biofilms adhered to framework alloys using in vitro denture plaque model

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説明

The purpose of this study was to perform a quantitative and qualitative evaluation of the biofilms formed on framework alloys in vitro. The biofilms formed by unfiltered fresh human saliva or Streptococcus mutans and/or Candida albicans on commercially pure titanium and gold-copper-platinum demonstrated higher amounts than other alloy and resin samples. In contrast, the silverpalladium-copper-gold showed a significantly decreased level of biofilm formation. Although the adhesion level of Streptococcus mutans on cobalt-chromium was high, that of Candida albicans was less extensive. A T-RFLP analysis and qualitative PCR of the microbes in the biofilms were performed. In a cluster analysis of all T-RFLP profiles, the cobalt-chromium pattern was integrated into one cluster. On qualitative PCR, the existence of microorganisms related to caries, preriodontitis and aspiration pneumonia was observed. Our results showed that the biofilm formation on each framework alloy was different in terms of both the quantity and quality.

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