Effect of feeding of Chlorella-extract supplement in diet on cultured yellow tail-I

NAKAGAWA H., INAZUKA Y., YAMASAKI S., HIRATA H., KASAHARA S.

doi:10.11233/aquaculturesci1953.30.67

In order to determine the effect of Chlorella-extract on growth and physiological condition of cultured yellow tail (Seriola quinqueradiata), yearling yellow tail (ca. 77g BW) were fed with raw fish (mainly mackerel) as control group. As experimental group, 0.3% of the extract was added to the above feed. At 3 months after feeding, the blood was withdrown by cardiac puncture and some hematological and serological parameters were analyzed as physiological indexes. 1) Chlorella-extract had no effect on both growth rate and feed conversion efficiency after 6 months feeding, but was effective on body color and figure which were similar with the wild. 2) While abrupt increase of hematocrit value was caused in control group by the blood sampling process. It means that resistance power of the fish subjected to the handling was restored by the Chlorella-extract. 3) Experimental group showed higher in serum protein content (Biuret method), serum lipid content (Sulfo-phospho-vanilline method), serum albumin content (Salting-out method), blood sugar (Glucose oxidase method), and blood lactic acid (Harrower and Brown method) than control. 4) Chlorella-extract feeding caused to depress lipoperoxide (Thiobarbituric acidfluorophotometric method) and serum lipid/serum protein ratio, and to increase in the serum lipoprotein of albumin fraction (Salting-out and cellulose acetate electrophoresis) . These changes of the parameters might reflect certain occurrence in physiological improvement as lipid metabolism by Chlorella-extract feeding.

Effect of feeding of <I>Chlorella</I>-extract supplement in diet on cultured yellow tail-I

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