Lipoprotein of Thoroughbred Horse by Density Gradient Ultracentrifugation

DOI
  • SAKO Toshinori
    Department of Veterinary Internal Medicine, Nippon Veterinary and Zootechnical College
  • HASEGAWA Shinogu
    Department of Veterinary Internal Medicine, Nippon Veterinary and Zootechnical College
  • KOYAMA Hidekazu
    Department of Veterinary Internal Medicine, Nippon Veterinary and Zootechnical College
  • TAKAGI Shigeyoshi
    Equine Research Institute, Japan Racing Association
  • MOTOYOSHI Shigekatsu
    Department of Veterinary Internal Medicine, Nippon Veterinary and Zootechnical College

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Other Title
  • 密度勾配遠心法によるサラブレッド種の血漿リポタンパク分画

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Abstract

To clarify lipid component and lipoprotein in blood plasma of Thoroughbred horses, investigation was performed by density gradient ultracentrifugation on blood plasma taken from horses of 16 stallions aged 6 to 20, 16 mares aged 5 to 12, and each 6 male or female horses aged 2-years-old. As a result, lipoproteins was separated into 3 main fractions in order of the density named high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) plus chylomicron (CM). The composition of these components in horses was as followed.<br>1) Among plasma lipids, total cholesterol (TC) values in the female were significantly higher than those of the male.<br>2) The lipid composition of HDL was observed nearly at the certain level appoximately 65% in phospholipids (PL), and 30% in TC among the male and female.<br>3) The values of TC, PL and total lipids (TL) in the LDL of the female were significantly higher than those of the male despite of showing a significant low PL/TC ratios (p<0.01) in the LDL and high percentage (p<0.01) in the male.<br>4) The concentration of PL and TC in the VLDL was maintained at certain level approximately 10% and VLDL was composed of triacylgylcerol (TG) rich component.<br>These results suggested that the high value of plasma TC in the female comparing with that in the male was resulted in increase of LDL and TC component. In addition, the high ratio of TC and low ratio of PL/TC despite of low value of TC in LDL in the male suggested that there were some factors affected on the composition of LDL in the male.

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