遠沈法によるワムシの定量

DOI 日本農学文献記事索引 Web Site 被引用文献7件 参考文献14件

書誌事項

タイトル別名
  • Determination of Marine Rotifer Biomass by Centrifugation.
  • エンチンホウ ニヨル ワムシ ノ テイリョウ

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抄録

For the reliable management of rotifer culture, it is most important to estimate the absolute quantities of food organism provided and rotifer biomass, accurately, simply and rapidly. In this study we tested the usefulness of centrifugation method to measure the biomass of Chlorella regularis and Brachionus rotundiformis as packed volume (PV, ml/l) with a graduated capillary tube (hematocrit tube) .<BR>The optimal conditions for centrifugation were first determined as 2808 G (R. C. F) and 30 minutes. Next, we compared the accuracy of estimation by the conventional direct-counting method under a microscope and by centrifugation using the same rotifer and Chlorella samples. As a result, the centrifugation method (coefficient of variation: 3.9% for Chlorella and 3.6% for rotifers) was found to be more accurate than the conventional counting method (coefficient of variation: 14.1% for Chlorella and 15.3% for rotifers) for estimating rotifer and Chlorella biomass in the medium.

収録刊行物

  • 水産増殖

    水産増殖 45 (2), 171-177, 1997

    日本水産増殖学会

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