Preparation of injectable 3D-formed β-tricalcium phosphate beadalginate composite for bone tissue engineering

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  • MATSUNO Tomonori
    Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University
  • HASHIMOTO Yoshiya
    Department of Biomaterials, Osaka Dental University
  • ADACHI Seita
    Department of Biomaterials, Osaka Dental University
  • OMATA Kazuhiko
    Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University
  • YOSHITAKA Yamauchi
    Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University
  • OZEKI Yasuyuki
    New Material Science Laboratory, Advance Ltd.,
  • UMEZU Yoshikazu
    New Material Science Laboratory, Advance Ltd.,
  • TABATA Yasuhiko
    Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University
  • NAKAMURA Masaaki
    Department of Biomaterials, Osaka Dental University
  • SATOH Tazuko
    Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University

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タイトル別名
  • Preparation of injectable 3D-formed .BETA.-tricalcium phosphate bead/alginate composite for bone tissue engineering

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説明

A novel, injectable bone tissue engineering material was developed that consisted of β-tricalcium phosphate (β-TCP) beads as the solid phase and alginate as the gel phase. To prepare the instantaneously formed composite scaffold, an aqueous calcium chloride solution was dried on the surface of β-TCP beads and crosslinked with an alginic acid sodium solution, thereby forming stable β-TCP beads and alginate gel which were injectable via a syringe. This biodegradable composite was a three-dimensional (3D) material that could be used as an injectable scaffold for bone tissue engineering. In particular, the composite with 2.0 wt% alginate concentration exhibited a compressive strength of 69 kPa in dry conditions, which was significantly higher than that exhibited by 1.0 wt%. Furthermore, mesenchymal stem cells (MSC) were 3D-cultured within the composite and then investigated for osteogenic markers. MSC-loaded composite was subjected to scanning electron microscope (SEM) examination and implanted subcutaneously for in vivo experiment. Results showed that the scaffold provided support for osteogenic differentiation. In light of the encouraging results obtained, this novel injectable composite material may be useful for bone tissue engineering.

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