Glycine 亜属植物の Kunitz トリプシンインヒビター様タンパク質の比較

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  • Comparative study of kunitz trypsin inhibitor like proteins in the subgenus Glycine.
  • Glycine アゾク ショクブツ ノ Kunitz トリプシン インヒビター

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KTILPs (Kunitz trypsin inhibitor like proteins) of wild soybeans (Glycine subgenus Glycine) are the antigenic relatives with KTI's found in cultivated soybeans (G. max). Seeds of wild soybeans contain two different KTILPS (S and F proteins) which migrate slow and fast on polyacrylamide gel electrophoresis (PAGE), respectively. The S proteins are single protein like KTI but the F proteins are composed of two different peptides (f1 and f2). They exhibit considerable variances of mobility (Rf value) on PAGE (Table 1), the ratios of S to F protein (Fig.1, Table 1) and the molecular sizes of S protein, f1 and f2 peptides on SDS-PAGE (Fig.3, 4). These three characteristics of KTILPs were compared in 22 lines of the five Glycine species (G.latifolia, G.tabacina, G.clandestina, G.canescens and G.tomentella) listed in Table 1. Seven migration patterns were distinguished by two dimensional gel electrophoresis consisting of PAGE and SDS-PAGE (Fig.3, 4). As G.latifolia and G. tabacina were separated into two (LAT A, B) and three (TAB A, B, C) subspecific groups, whereas G.clandestina formed a homogenous entity.

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