Possible Role of Microalga <I>Nannochloropsis</I> in Controlling <I>Vibrio</I> Species in Fish Larva Rearing Water

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  • 仔稚魚飼育水での微細藻類ナンノクロロプシスによるビブリオ属細菌の制御
  • Possible role of microalga Nannochloropsis in controlling Vibrio species in fish larva rearing water

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Abstract

We performed duplicate microcosm experiments to determine Vibrio species dynamics over 13 days in enriched natural seawater inoculated with Nannochloropsis. Chlorophyll a concentrations in Tanks 1 and 2 ranged from 8.71 to 235μg/l and 10.9 to 255μg/l, respectively; peak values were recorded on Day 6 in both tanks. Culturable Vibrio abundance on TCBS agar decreased from 180 CFU/ml on Day 2 to 50 CFU/ml on Day 5 in Tank 1, and from 120 CFU/ml on Day 1 to 20 CFU/ml on Day 6 in Tank 2. Total Vibrio abundance estimated by qPCR ranged from 3.2×102 to 2.6×104 cells/ml in Tank 1 and from 2.2×102 to 1.6×104 cells/ml in Tank 2. Interestingly, after the peak of chlorophyll a concentration in both tanks, no culturable Vibrio and only a few total Vibrio were detected. PCR-DGGE analysis showed that the Vibrio community was unexpectedly diverse at the onset, but the structure became simpler over time with the increasing chlorophyll a concentration. Hence, the addition of Nannochloropsis to larval-rearing water may control Vibrio species growth, a key factor responsible for the survival and growth of fish larvae.

Journal

  • Aquaculture Science

    Aquaculture Science 59 (3), 451-458, 2011

    Japanese Society for Aquaculture Science

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