Evaluation of an Experimental Immersion Infection Method with <I>Tenacibaculum maritimum</I> in Japanese Flounder <I>Paralichthys olivaceus</I>

DOI JASI Web Site 28 References

Bibliographic Information

Other Title
  • ヒラメ滑走細菌症の浸漬法による実験感染条件について
  • Evaluation of an experimental immersion infection method with Tenacibaculum maritimum in Japanese flounder Paralichthys olivaceus

Search this article

Abstract

Necessary condition to perform experimental infection with Tenacibaculum maritimum was investigated. A shaking culture of the bacterium for 48 h was used to infect Japanese flounder Paralichthys olivaceous. First, three infection methods, intraperitoneal (IP) injection and two immersion methods were compared. IP injected flounder with T. maritimum at doses of 104.82 or 104.92 CFU/fish did not exhibit high mortality or typical symptoms. “Immersion and dilution method”, where immersion-infected fish at doses 105.81 or 105.91 CFU/ml were successively reared in the same tank supplying fresh rearing water, resulted in higher mortality than “immersion and transfer method”, where immersion-infected fish were transferred to another rearing tank. Using immersion and dilution method, stable mortality occurred between 17°C and 26°C but not below 17°C or over 26°C. Too much diluted bacterial culture with sea water did not yield high mortality even when the immersion water contained enough bacterial concentration. This suggests extracellular substance contains virulence factor. T. maritimum was detected on the body surface of infected fish but not in the gills or kidney. In conclusion, the immersion and dilution method is an adequate infection method for this disease. In order to achieve satisfactory infection, the precise management of water temperature and the dilution rate of bacterial culture are required.

Journal

  • Aquaculture Science

    Aquaculture Science 58 (4), 481-489, 2010

    Japanese Society for Aquaculture Science

References(28)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top