Classification and identification of bunching onion (Allium fistulosum) varieties based on SSR markers

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  • Tsukazaki Hikaru
    National Institute of Vegetable and Tea Science (NIVTS)
  • Honjo Masanori
    Graduate School of Life and Environmental Sciences, University of Tsukuba Present address: National Agricultural Research Center for Tohoku Region (NARCT)
  • Yamashita Ken-ichiro
    National Institute of Vegetable and Tea Science (NIVTS)
  • Ohara Takayoshi
    National Institute of Vegetable and Tea Science (NIVTS)
  • Kojima Akio
    National Institute of Vegetable and Tea Science (NIVTS)
  • Ohsawa Ryo
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Wako Tadayuki
    National Institute of Vegetable and Tea Science (NIVTS)

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Abstract

We investigated the correspondence between classifications based on simple sequence repeat (SSR) markers and on morphological traits for 30 bunching onion (Allium fistulosum L.) varieties. We also examined applicability of an assignment test for variety identification in bunching onion. Cluster analysis based on the allele frequency data at 29 SSR loci classified most of the varieties except for ‘Iwatsuki-2’ into the predicted variety groups, ‘Kaga’, ‘Senju’ or ‘Kujo’, which were categorized based on morphological traits. Although ‘Iwatsuki-2’ has been regarded as a member of ‘Kaga’ group, molecular data suggested the variety belonging to ‘Kujo’ rather than ‘Kaga’. In the assignment test at the individual level, 89.1% of the individuals were assigned to their original variety. When the assignment was conducted based on groups each consisting of four individuals, the percentage of correct assignments was considerably improved (99.3%). These results suggested that the assignment test approach will be useful for variety identification in allogamous bunching onions, which have large within-variety genetic diversity. On the other hand, it was also suggested that sampling of true source varieties will be fundamental to avoid misjudgment.<br>

Journal

  • Breeding Science

    Breeding Science 60 (2), 139-152, 2010

    Japanese Society of Breeding

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