Polyacrylamide gel electrophoresis of S-RNase fragments for identification of S-genotypes of Japanese pear (Pyrus pyrifolia)
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- Kato Masaki
- Graduate School of Horticulture, Chiba University
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- Kato Shu
- Chiba Prefectural Agriculture and Forestry Research Center
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- Sassa Hidenori
- Graduate School of Horticulture, Chiba University
書誌事項
- タイトル別名
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- Polyacrylamide gel electrophoresis of <i>S-RNase</i> fragments for identification of <i>S</i>-genotypes of Japanese pear (<i>Pyrus pyrifolia</i>)
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抄録
Japanese pear (Pyrus pyrifolia) exhibits gametophytic self-incompatibility (GSI) controlled by a complex and multiallelic S locus. The pistil-part product of the S locus is the polymorphic ribonuclease, S-RNase. Information on S-genotypes is important for the production and breeding of Japanese pears. Molecular analyses of S-genotypes of Japanese pear have been conducted with the CAPS (cleaved amplified polymorphic sequence) system; PCR amplification of S-RNase fragments by a common primer pair followed by digestion with restriction enzymes each of which cleaves a specific S haplotype. Here, we show that the separation of S-RNase fragments by polyacrylamide gel electrophoresis (PAGE) distinguishes four out of nine S haplotypes of Japanese pear without restriction digestion. S3-, S5-, S6- and S8-RNases were identified as distinct bands by PAGE. S3- and S5-RNases were separated by PAGE despite their identical fragment sizes. Using this system, three Japanese pear lines with unknown S-genotypes were analyzed. The newly determined S-genotypes of the lines were confirmed by CAPS analysis.
収録刊行物
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- Breeding Science
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Breeding Science 62 (4), 348-351, 2012
日本育種学会
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詳細情報 詳細情報について
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- CRID
- 1390001204723112832
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- NII論文ID
- 130004146350
- 10031131504
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- NII書誌ID
- AA11317194
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- COI
- 1:STN:280:DC%2BC3szitVCitw%3D%3D
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- ISSN
- 13473735
- 13447610
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- NDL書誌ID
- 024151353
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- PubMed
- 23341749
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可