Persistent C genome chromosome regions identified by SSR analysis in backcross progenies between <i>Brassica juncea</i> and <i>B. napus</i>

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  • Tsuda Mai
    Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences (NIAS)
  • Okuzaki Ayako
    Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences (NIAS)
  • Kaneko Yukio
    Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University
  • Tabei Yutaka
    Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences (NIAS)

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  • Persistent C genome chromosome regions identified by SSR analysis in backcross progenies between Brassica juncea and B. napus

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Abstract

Given that feral transgenic canola (Brassica napus) from spilled seeds has been found outside of farmer’s fields and that B. juncea is distributed worldwide, it is possible that introgression to B. juncea from B. napus has occurred. To investigate such introgression, we characterized the persistence of B. napus C genome chromosome (C-chromosome) regions in backcross progenies by B. napus C-chromosome specific simple sequence repeat (SSR) markers. We produced backcross progenies from B. juncea and F1 hybrid of B. juncea × B. napus to evaluate persistence of C-chromosome region, and screened 83 markers from a set of reported C-chromosome specific SSR markers. Eighty-five percent of the SSR markers were deleted in the BC1 obtained from B. juncea × F1 hybrid, and this BC1 exhibited a plant type like that of B. juncea. Most markers were deleted in BC2 and BC3 plants, with only two markers persisting in the BC3. These results indicate a small possibility of persistence of C-chromosome regions in our backcross progenies. Knowledge about the persistence of B. napus C-chromosome regions in backcross progenies may contribute to shed light on gene introgression.

Journal

  • Breeding Science

    Breeding Science 62 (4), 328-333, 2012

    Japanese Society of Breeding

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