Effects of seed storage and pre-soaking dry treatment on chromosome aberrations in mitosis of Allium fistulosum L.

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  • ネギ種子の貯蔵と浸漬乾燥処理により誘起された体細胞染色体異常
  • ネギ シュシ ノ チョゾウ ト シンセキ カンソウ ショリ ニ ヨリ ユウキサ

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Abstract

Effects of seed storage and exposure of pre-soaked seeds to dry treasment (50°C) on chromosome aberrations were examined. Stored seeds of Allium fistulosum L. cv. Kujohosonegi and Ishikura-ippon-futonegi which was obtained from Mikado Seed Co., Ltd. were used in the present investigation. Chromosome aberrations at mitotic meta- and anaphase were examined in the primary root tips of germinated seeds. High frequency of spontaneous chromosome aberrations (mean 51%) was observed in the stored seeds, whose cytological characteristics were remarkable fragmentations of the chromosome-type. From this result, the most aberrations were thought to be induced before DNA synthesis stage. The present study showed a positive correlation between the frequency of spontaneous chromosome aberrations and the number of days to germination, and a negative correlation between the former and the length of the primary seedling root.

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