Identification of plant residual substances in the feces of <i>Apodemus speciosus</i> and <i>A. argenteus</i> by chloroplast <i>rbcL</i> gene sequences

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  • Shirako Tomoyasu
    Chubu University, Graduate School of Bioscience and Biotechnology
  • Aichi Makiko
    Chubu University, Graduate School of Bioscience and Biotechnology
  • Ueno Kaoru
    Chubu University, Graduate School of Bioscience and Biotechnology
  • Minami Motoyasu
    Chubu University, Graduate School of Bioscience and Biotechnology

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  • 葉緑体DNA <i>rbcL</i>遺伝子によるアカネズミおよびヒメネズミの糞中植物種残渣推定
  • 葉緑体DNA rbcL遺伝子によるアカネズミおよびヒメネズミの糞中植物種残渣推定
  • ヨウリョクタイ DNA rbcL イデンシ ニ ヨル アカネズミ オヨビ ヒメネズミ ノ フン チュウ ショクブツシュザンサスイテイ

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Abstract

A molecular identification method for residual food substances in the feces of Apodemus speciosus and A. argenteus was evaluated. We collected the feces in Mt. Miroku, Aichi Pref., Japan, using Sherman traps during 2009–2010. To understand plant food utilization of both mice species in the research area, total DNA was extracted from fecal samples and the chloroplast rbcL gene region (262 bp) was amplified by PCR. We successfully amplified and obtained 262 bp DNA sequences by subcloning. Homology searches of the sequences via BLAST were then performed to identify food resources from the feces of both species. Lactuca serriola (Asteraceae), Wisteria floribunda (Fabaceae), and Cerasus jamasakura var. jamasakura (Rosaceae) were confirmed from the feces of A. speciosus. In addition, four plant species, L. serriola, Chamaecyparis obtuse var. obtusa (Cupressaceae), Pieris japonica var. japonica (Ericaceae), and C. jamasakura var. jamasakura were also found in the feces of A. argenteus. The present method using fecal DNA and molecular techniques was well suited for the identification of plant materials considering the diet of small rodents.

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