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説明
For AFLP analysis of grapes, three different concentrations of restriction enzymes were examined to reduce artifact bands due to incomplete digestion or star activity of the restriction enzymes. Differences among band intensities were neglected, and all the detected bands were used in the analysis. A phenogram of 12 cultivars of Vitis vinifera, two cultivars of V.labrusca and two wild species, V.ficifolia var.lobata (V.thunbergii, Ebizuru) and V.coignetiae (Yamabudou) was obtained by UPGMA (unweighted pair group method arithmetic averages) based on the percentage of unshared AFLP bands of each sample pair, using four primer pairs. This phenogram revealed a clear separation of the tested species and proles with high bootstrap probabilities, except for the clusters with a longer phenetic distance in the Oriental cultivars of V.vinifera. The cluster of Occidental cultivars split into a cluster consisting of Cabernet Sauvignon and Cabernet franc, and another cluster consisting of Pinot noir, Pinot blanc, Chardonnay and Riesling. Among the Oriental cultivars, two Japanese cultivars, Koshu and Koshu-Sanjaku, formed a cluster. Thus, this phenogram agreed well with the classification based on morphological properties and geographical location.
収録刊行物
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- Ｂｒｅｅｄｉｎｇ Ｓｃｉｅｎｃｅ
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Ｂｒｅｅｄｉｎｇ Ｓｃｉｅｎｃｅ 50 (1), 53-57, 2000
日本育種学会
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詳細情報 詳細情報について
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- CRID
- 1390001204724294144
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- NII論文ID
- 130004264197
- 110001808038
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- NII書誌ID
- AA11353132
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- ISSN
- 13473735
- 13447610
- http://id.crossref.org/issn/13447610
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- NDL書誌ID
- 5302796
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
