プロテオーム解析に基づく内耳特異的蛋白抗原の分離回収

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  • Recovery of inner ear specific protein based on proteome profiling

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In order to establish experimental inner ear specific autoimmune labyrinthitis in mice, the present study was designed to recover inner ear specific proteins from crude inner ear proteins by SDS-PAGE technique and proteome profiling. Thirty partitioned inner ear proteins according to molecular size were harvested through elution of crude inner ear antigens on Whole Gel Eluter. Serum from mice sensitized with 19 out of 30 partitions produced antibodies against bovine inner ear proteins by western blotting and 7 positive different bands were recognized. Proteome profiling of the several partitions that demonstrated clear positive bands were selectively carried out. Partition number 3, 10 and 11 hit only COCH protein. Partition number 5 and 8 hit COCH protein together with non specific inner ear proteins. Partition number 18, 22 and 24 hit non specific inner ear proteins. The present study succeeded in isolation of inner ear specific proteins, suggesting that development of inner ear specific autoimmune animal model is possible to elucidate pathogenesis of autoimmune inner ear disease.

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