Potassium current properties in isolated Deiters' cells from guinea-pig cochlea

DOI 20 References
  • Nawate Ayako
    Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University Department of Otorhinolaryngology, Hamanomachi Hospital
  • Kimitsuki Takashi
    Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University
  • Tamae Akihiro
    Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University
  • Matsumoto Nozomu
    Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University
  • Shibata Shumei
    Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University
  • Ohashi Mitsuru
    Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University
  • Komune Shizuo
    Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University

Bibliographic Information

Other Title
  • モルモット蝸牛ダイテルス細胞のＫ電流の電気生理学的特性

Search this article

Description

Deiters' cells in the organ of Corti possess gap junctions that contribute to the K recycling system in the cochlea. Among the supporting cells, Deiters' cells are in close contact at the apical pole and at the basal pole to the outer hair cells, so Deiters' cells play an important role in the K recycling system. We studied the properties of K currents in isolated Deiters' cells from guinea-pig cochlea. Deiters' cells were isolated by trypsin and mechanical triturations, and could be identified by those morphologic characteristics. The K currents were measured by the conventional mode of patch electrode voltage-clamp technique. In standard extracellular solutions, voltage steps in the negative direction yielded very small currents while steps in the positive direction yielded very large currents, suggesting properties of outward rectification. The conductance of peak currents at +100mV was 26.2 nS. The currents demonstrated a fast activation (within 10 ms) and slower inactivation (in the range of seconds). Temperature-dependence of K current kinetics was evaluated by comparing that at room temperature with that at 36°C The amplitude of K currents increased at 36°C and both the rates of activation and inactivation increased at 36°C. Q10 of the activation rate was 1.78 and that of inactivation was 1.51.

Journal

  • Otology Japan

    Otology Japan 20 (1), 1-6, 2010

    Japan Otological Society

References(20)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top