菌体内毒素のマウスγδ型Ｔリンパ球に対するマイトジェン作用

DOI 参考文献17件

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タイトル別名
  • Mitogenic activity of bacterial lipopolysaccharide on the .GAMMA..DELTA. T lymphocyte.

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In vitro proliferative responses of T lymphocytes to bacterial lipopolysaccharides (LPS) were examined by determining the uptake of tritiated thymidine (3H-TdR) into cells. T lymphocyte populations were taken and purified from proteose peptoneinduced peritoneal exudate cell (PEG), spleen and thymus of C3H/HeN or C3H/HeJ mice. LPS were prepared from Salmonella typhimurium, Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum by the phenolwater proceduce.<BR>The T lymphocytes taken from PEG (PEG-T) of C3H/HeN mice showed an increased 3H-TdR uptaken in response to LPS from S. typhimurium (S. t-LPS), while PEG-T of G3H/HeJ, splenic T and thymic T lymphocyte of C3H/HeN mice did not respond to S. t-LPS. Other LPS also have a mitogenic ability to PEG -T lymphocytes too, while those of S. t-LPS and P. i-LPS were higher than that of F. n-LPS or P. g-LPS. The response of PEG from C3H/HeN mice to S. t-LPS was abolished, when the PEG-T were pretreated with anti-Thy-1 orγδTCR antibody plus complement (c), but not anti-αβTCR antibody plus C. The thymic T lymphocytes did not show any increase of 3H-TdR uptake in response to LPS or anti-γδ TCR antibody. However, obvious uptake did occur when the cells were stimulated with LPS and anti-γδ TCR antibody together.<BR>The results suggest that LPS has a mitogenic ability to a T lymphocytes population bearing γδ TCR in PEG that had been stimulated previously by proteose peptone.

収録刊行物

  • 炎症

    炎症 15 (1), 49-54, 1995

    日本炎症・再生医学会

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