Involvement of Two Cytosolic Enzymes and a Novel Intermediate, 5′-Oxoaverantin, in the Pathway from 5′-Hydroxyaverantin to Averufin in Aflatoxin Biosynthesis
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- SAKUNO Emi
- Faculty of Agriculture, Tottori University National Food Research Institute
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- NAKAJIMA Hiromitsu
- Faculty of Agriculture, Tottori University
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- YABE Kimiko
- National Food Research Institute
書誌事項
- タイトル別名
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- Involvement of two cytosolic enzymes and a novel intermediate, 5'-oxoaverantin, in the pathway from 5'-hydroxyaverantin to averufin in aflatoxin biosynthesis
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説明
In aflatoxin biosynthesis 5'-hydroxyaverantin (HAVN) is converted to averufin (AVR). We demonstrated that this step is composed of two enzymatic steps: an enzyme, HAVN dehydrogenase, catalyzes the reaction from HAVN to a new intermediate and another new enzyme catalyzes the following reaction from the intermediate to AVR. The intermediate was assigned to be 5'-oxoaverantin (OAVN) by spectroscopic methods. We purified HAVN dehydrogenase and OAVN cyclase from the cytosol fraction of A. parasiticus and characterized. MALDI-TOFMS analysis of tryptic peptides of the purified HAVN dehydrogenase revealed that this enzyme is encoded by adhA gene in the aflatoxin gene cluster of A. parasiticus.
収録刊行物
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- マイコトキシン
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マイコトキシン 2003 (Suppl3), 349-352, 2003
日本マイコトキシン学会