Development of a highly sensitive yeast bioassay for trichothecene detection
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- TANAKA Akira
- Graduate School of Engineering, Toyo University
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- YAMANE Yumi
- Graduate School of Engineering, Toyo University
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- KOMIYA Yohei
- Graduate School of Engineering, Toyo University
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- YAMAUCHI Kohta
- Graduate School of Engineering, Toyo University
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- SUGIYAMA Tomoki
- Graduate School of Engineering, Toyo University
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- ECHIGO Akinobu
- Bio-Nano Electronics Research Centre, Toyo University
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- USAMI Ron
- Bio-Nano Electronics Research Centre, Toyo University
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- YOSHIDA Yasuhiko
- Graduate School of Engineering, Toyo University Bio-Nano Electronics Research Centre, Toyo University
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- ABE Fumiyoshi
- Department of Chemistry and Biological Science, College of Science and Engineering
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- MINEGISHI Hiroaki
- Bio-Nano Electronics Research Centre, Toyo University
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- TAKAHASHI-ANDO Naoko
- Graduate School of Engineering, Toyo University Bio-Nano Electronics Research Centre, Toyo University
Bibliographic Information
- Other Title
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- 遺伝子破壊酵母を用いたトリコテセン簡易検出法
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Abstract
Trichothecenes are mycotoxins produced by Fusarium and other genera that can cause serious health problems in humans and livestock. We previously reported 22 trichothecene resistance genes in Saccharomyces cerevisiae, whose deletion conferred high sensitivity to trichothecenes. In this study, we constructed various multiple gene deletion mutants of these resistance genes to develop a sensitive yeast bioassay system for trichothecenes. Among the double and triple null mutants, pdr5Δ erg6Δ rpb4Δ cells showed high sensitivity to T-2 toxin and deoxynivalenol under optimized culture conditions (IC50 values of 1.5 ng/mL and 1.5 μg/mL, respectively). The disc diffusion test also confirmed the high sensitivity of this mutant to the trichothecenes. This detection system was more sensitive than any other yeast bioassay previously developed. The pdr5Δ erg6Δ rpb4Δ cells detected 1.0 ppm deoxynivalenol in wheat flour and 1.1 ppm deoxynivalenol in wheat grain, which are the advisory level for deoxynivalenol in final wheat products in US and the provisional level for wheat grain in Japan, respectively. Here, we report an easy, inexpensive, and highly sensitive yeast bioassay system to detect trichothecenes.
Journal
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- JSM Mycotoxins
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JSM Mycotoxins 63 (2), 161-170, 2013
Japanese Society of Mycotoxicology
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Details 詳細情報について
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- CRID
- 1390001204786466688
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- NII Article ID
- 130003384017
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- NII Book ID
- AA12721071
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- ISSN
- 18810128
- 02851466
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- NDL BIB ID
- 027209227
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed