Effects of Perfusate Flow and Viscosity on Hypoxic Pulmonary Vasoconstriction in Rat Pulmonary Artery

DOI Web Site 20 References
  • Minami Kazuhiro
    Department of Environmental Symbiotic Studies, Graduate School of Agriculture, Tokyo University of Agriculture
  • Kashimura Osamu
    Faculty of International Agriculture and Food Studies, Tokyo University of Agriculture

Bibliographic Information

Other Title
  • ラットにおける低酸素性肺血管収縮に流量および粘性が与える影響
  • ラット ニ オケル テイサンソセイ ハイケッカン シュウシュク ニ リュウリョウ オヨビ ネンセイ ガ アタエル エイキョウ

Search this article

Description

Background: Hypoxic pulmonary vasoconstriction (HPV) is one cause of mountain sickness such as lung edema. However, few studies have examined the effects of increased pulmonary blood flow and viscosity on HPV.<br> Objectives: We postulated that increasing blood flow and viscosity inhibit HPV during exercise. We therefore measured pulmonary arterial pressure with stepwise changes of perfusate flow and viscosity under conditions of normoxia (20% O2) and hypoxia (0% O2) in isolated perfused rat arteries. We used a pharmacological inhibitor (L-NAME) to assess whether nitric oxide synthase contributes to flow- and viscosity-induced inhibition of HPV. Methods: Isolated pulmonary arteries were attached to the blood vessel perfusion system and pulmonary arterial pressure was recorded. The perfusate was physiological salt solution (PSS) without or with 5% Dextran (PSSD). The flow rate of the perfusate was increased from 0.03 to 0.0902 mL/g/min in stepwise increments of 0.02 mL/g/min over 5 min during exposure to normoxia and hypoxia.<br> Results: The increase in flow and viscosity of both perfusates did not affect HPV. However, increasing flow and viscosity of each perfusate increased HPV after L-NAME administration. Conclusion: We found that HPV might be inhibited by NO release induced by shear stress such as pulmonary blood flow and viscosity, in rat isolated pulmonary arteries.<br>

Journal

References(20)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top