書誌事項
- タイトル別名
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- A simple enzymatic method for isolation of rat hepatocytes and its application
- カン サイボウ ノ カンタン ナ ブンリホウ ノ カイハツ ト ソノ バイヨウ
- Effect of prednisolone on carbohydrate and protein metabolism in rat hepatocyte
- 副腎皮質ホルモン投与時の培養肝細胞の代謝
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1) Procedure for isolation of rat hepatocytes: About 30ml of 0.05% bacterial collagenase in Ca-free Hanks' solution was directly injected into the aseptically removed liver tissue through tuberculine needle at a speed of 20ml/min. After a few minutes of injection, liver tissue was torn into small pieces by two forcepts and then filtered through a platinum sieve with 150 meshes. Filtrate was centrifuged several times at a very low speed (200rpm). The final residue was suspended in Eagle's minimum essential medium containing 20% fetal calf serum and used for primary culture. The yield of isolated hepatocyte was (7.86±4.24)×106/g wet liver and contamination by Kupffer cells was less than 1%. More than 95% of cells excluded trypan blue.<BR>2) Effect of prednisolone on gluconeogenesis and protein biosynthesis: Gluconeogenesis in cultured hepatocytes from alanine or lactate added to the culture media was enhanced by 100μg/dish of prednisolone. Contrary to it, incorporation of 14C-leucine or 3H-proline into the newly synthesized protein was inhibited by the same amount of prednisolone. Radioactivity of 3Hhydroxyproline was detected and the nature of the protein containing hydroxyproline was discussed.
収録刊行物
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- 肝臓
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肝臓 19 (4), 327-331, 1978
一般社団法人 日本肝臓学会
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詳細情報 詳細情報について
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- CRID
- 1390001204795782272
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- NII論文ID
- 130000874347
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- NII書誌ID
- AN00047770
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- ISSN
- 18813593
- 04514203
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- NDL書誌ID
- 1937952
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可