書誌事項
- タイトル別名
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- A New type of Protein Profiling System using CPDIB (Crude Protein Direct Blotting) assay
- ソタンパク チョクセツコソウホウ ニ ヨル タンパクシツ プロファイリング システム ノ カイハツ
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抄録
Proteome research in the medical field is expected to accelerate the understanding of disease mechanism, and to create new therapeutic and diagnostic concept. For protein profiling, this paper proposes a new methodology named CPDIB (Crude Protein Direct Blotting). In CPDIB assay, crude protein sample is directly immobilized on a hydrophobic membrane, and then the expression of molecules in the sample are analyzed quantitatively by using a special device, where the membrane is used as a field of immune reaction. The over-all structure of the special device is based on the conventional slot-blot device. Mechanical improvement in the air-tightness of the case holding the membrane realizes the direct blotting and results in the high performance of the stability in the immune reaction. This paper shows the mechanical structure of the membrane assembly called ImmobiChip and the evaluation of the protein profiling and its reproducibility.
収録刊行物
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- 精密工学会誌
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精密工学会誌 69 (12), 1796-1800, 2003
公益社団法人 精密工学会
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詳細情報 詳細情報について
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- CRID
- 1390001204796488832
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- NII論文ID
- 110001368399
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- NII書誌ID
- AN1003250X
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- ISSN
- 1882675X
- 09120289
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- NDL書誌ID
- 6797018
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可