A New type of Protein Profiling System using CPDIB (Crude Protein Direct Blotting) assay

Bibliographic Information

Other Title
  • 粗タンパク直接固相法によるタンパク質プロファイリングシステムの開発
  • ソタンパク チョクセツコソウホウ ニ ヨル タンパクシツ プロファイリング システム ノ カイハツ

Search this article

Abstract

Proteome research in the medical field is expected to accelerate the understanding of disease mechanism, and to create new therapeutic and diagnostic concept. For protein profiling, this paper proposes a new methodology named CPDIB (Crude Protein Direct Blotting). In CPDIB assay, crude protein sample is directly immobilized on a hydrophobic membrane, and then the expression of molecules in the sample are analyzed quantitatively by using a special device, where the membrane is used as a field of immune reaction. The over-all structure of the special device is based on the conventional slot-blot device. Mechanical improvement in the air-tightness of the case holding the membrane realizes the direct blotting and results in the high performance of the stability in the immune reaction. This paper shows the mechanical structure of the membrane assembly called ImmobiChip and the evaluation of the protein profiling and its reproducibility.

Journal

References(7)*help

See more

Details 詳細情報について

Report a problem

Back to top