A New type of Protein Profiling System using CPDIB (Crude Protein Direct Blotting) assay
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- KOBAYASHI Hironori
- シスメックス(株) 鳥取大学大学院工学研究科
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- KAWASAKI Yuko
- シスメックス(株)
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- ISHIHARA Hideki
- シスメックス(株)
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- YAMAGATA Koichi
- シスメックス(株)
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- MIZUMOTO Hiroshi
- 鳥取大学工学部
Bibliographic Information
- Other Title
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- 粗タンパク直接固相法によるタンパク質プロファイリングシステムの開発
- ソタンパク チョクセツコソウホウ ニ ヨル タンパクシツ プロファイリング システム ノ カイハツ
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Abstract
Proteome research in the medical field is expected to accelerate the understanding of disease mechanism, and to create new therapeutic and diagnostic concept. For protein profiling, this paper proposes a new methodology named CPDIB (Crude Protein Direct Blotting). In CPDIB assay, crude protein sample is directly immobilized on a hydrophobic membrane, and then the expression of molecules in the sample are analyzed quantitatively by using a special device, where the membrane is used as a field of immune reaction. The over-all structure of the special device is based on the conventional slot-blot device. Mechanical improvement in the air-tightness of the case holding the membrane realizes the direct blotting and results in the high performance of the stability in the immune reaction. This paper shows the mechanical structure of the membrane assembly called ImmobiChip and the evaluation of the protein profiling and its reproducibility.
Journal
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- Journal of the Japan Society for Precision Engineering
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Journal of the Japan Society for Precision Engineering 69 (12), 1796-1800, 2003
The Japan Society for Precision Engineering
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Details 詳細情報について
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- CRID
- 1390001204796488832
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- NII Article ID
- 110001368399
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- NII Book ID
- AN1003250X
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- ISSN
- 1882675X
- 09120289
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- NDL BIB ID
- 6797018
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed