An investigation on pathological mechanism of staphylococcal scalded skin syndrome (SSSS). III An immuno-electron microscopic study of organ cultured epidermis with exfoliative toxin.

DOI

Bibliographic Information

Other Title
  • ブドウ球菌熱傷様皮膚症候群（ＳＳＳＳ）の発症病理に関する研究　ＩＩＩ　　Ｅｘｆｏｌｉａｔｉｖｅ　ｔｏｘｉｎ添加ヒト表皮器官培養の免疫電顕的観察
  • III An immuno-electron microscopic study of organ cultured epidermis with exfoliative toxin
  • 第3報Fxfoliative toxin添加ヒト表皮器官培養の免疫電顕的観察

Abstract

The purpose of the present study is to investigate the action site of exfoliative toxin (ET) in the epidermis by immuno-electron microscopy using protein-A colloidal gold complex method. After S. aureus strain ZM was cultured, ET was separated by modified Kondo's method. Anti-ET serum was obtained by immunization of rabbis with purified ET mixed with Freund complete adjuvant. Purified ET was applied to the normal human epidermal organ culture which was incuvated for 1.5, 2. 5 and 3.5 hours at 37°C, and specimens were prepared for light and transmission electron microscopy, immuno-electron microscopy, and indirect immunofluorescence microscopy. By light microscopy intraepidermal cleavage was observed at the level of the granular layer after 1.5 hours and extended to the upper spinous layer after 3.5 hours. By transmission electron microscopy, desmosomes were seen separated and the half-desmosome-like structure and tonofilament retracted into cytoplasm as seen in SSSS. By immunhfluorescence microscopy, intercellular fluorescence was first observed in the basal layer, and then in spinous and granular layers according to the time course. When reached the granular layer, acantholysis occurred. Acantholysis was also observed at 0°C. By immuno-electron microscopy, gold particles were mainly seen in the outer leaflet of desmosomes, but sometimes in the intercellular contact layer, attachment plaque and tonofilaments inserting insertingto desmosomes. From the present observation, it is likely that ET attaches first to the outermembrane component of desmsomes.

Journal

  • Skin research

    Skin research 29 (1), 20-37, 1987

    Meeting of Osaka Dermatological Association

Keywords

Details 詳細情報について

  • CRID
    1390001204798583168
  • NII Article ID
    130004045069
  • DOI
    10.11340/skinresearch1959.29.20
  • ISSN
    1884541X
    00181390
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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