ANTICANCER DRUG SENSITIVITY AND GENE ANALYSIS IN IRRADIATED GLIOBLASTOMA

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  • 放射線照射glioblastomaにおける抗癌剤感受性と遺伝子解析
  • ホウシャセン ショウシャ glioblastoma ニ オケル コウガンザイ カンジュセイ ト イデンシ カイセキ

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Abstract

Glioblastoma is a tumor that is difficult to treat radically by surgery alone; presently, surgery, radiotherapy, and chemotherapy are the combined treatment of choice. In general, postoperative radiotherapy and chemotherapy are performed concurrently, but this is not a radical treatment. Using human glioblastoma cell lines (T98G and A172 cells) from the Japanese Cancer Resources Bank, irradiated glioblastoma cells were prepared under the same conditions as actual clinical therapy. To these irradiated cells, 5-fluorouracil was administered and anticancer drug sensitivity was investigated in relation to the timing of the radiotherapy. In addition, the mRNA levels of TS, DPD, and OPRT, genes that can serve as biomarkers for 5-fluorouracil, were measured to investigate anticancer drug sensitivity and gene expression. When compared to unirradiated cells and 60-Gy-irradiated cells, the cell proliferation rate for 30-Gy-irradiated cells tended to be higher and 5-fluorouracil sensitivity was the highest for the 30-Gy-irradiated cells. In other words, from the viewpoint of antitumor effects, the results suggest that the antitumor effects of sequential therapy in which radiotherapy was followed by chemotherapy were greater than those of concurrent radiotherapy and chemotherapy. A spaces needed have TS was the lowest in the 30-Gy-irradiated cells and the highest in the unirradiated cells. Because anticancer drug sensitivity was the highest with 30Gy, patients with high TS levels are thought to be resistant to 5-fluorouracil, while those with low TS levels are believed to be sensitive to the anticancer drug. The mRNA levels of DPD and OPRT did not correlate with 5-fluorouracil sensitivity. The results suggest that TS may become a biomarker for 5-fluorouracil.

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