EVALUATION OF NUCLEOLAR ORGANIZER REGIONS IN GYNECOLOGICAL CYTOLOGY

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  • SATO Ikuo
    2nd Department of Pathology, Showa University School of Medicine
  • KAWASE Norio
    2nd Department of Pathology, Showa University School of Medicine
  • KUSHIMA Tomoki
    2nd Department of Pathology, Showa University School of Medicine
  • SHIOKAWA Akira
    2nd Department of Pathology, Showa University School of Medicine
  • OOTA Shuichi
    2nd Department of Pathology, Showa University School of Medicine
  • MATSUI Naruaki
    Department of Pathology, Kawasaki Kyodo Hospital

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  • 婦人科細胞診におけるAgNORs染色法の検討

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Abstract

Recently, reseachers have been studying silver stained nucleolar organizing regions (AgNORs), loops of DNA that transcribe into ribosomal RNA. The number of AgNORs is thought to reflect cellular proliferative activity, but studies of AgNORs in squamous cells of uterine cervical areas are rarely reported, especially in cytology. In the present study, we tried special methods of sampling, smearing, and staining squamous cervical cells, and succeeded in acquiring samples for cytodiagnosis. Next, we tried to count AgNORs in 100 cells from uterine cervices of women, who requested examination of cervical smears in our hospital. The mean AgNORs counts of total squamous cells were closely related to the mean AgNOR counts in intermediate cells. As aging progressed, middle or large sized AgNOR counts increased, mainly in intermediate cells. Pregnancy did not affect the count. In women who used estrogen, large sized AgNOR counts increased, mainly in intermediate cells. In the secretory phase of the menstrual cycle, small sized AgNOR counts increased, mainly in superficial and intermediate cells. During inflammation, middle or large sized AgNOR counts increased. In uterine cervical squamous cell cancer, AgNOR counts increased markedly, compared to benign cases; and AgNOR counts in parabasal cells also increased. The results suggest that AgNOR counts may be useful in cytodiagnosis of the uterine cervical area.

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