無麻酔, 無拘束動物の脳幹単一ニューロン放電の記録法

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  • THE METHODS FOR RECORDING SINGLE NEURON ACTIVITY FROM BRAIN STEM IN UNANESTHETIZED AND UNRESTRAINED ANIMALS
  • ム マスイ ム コウソク ドウブツ ノ ノウカン タンイツ ニューロン ホウデ

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The particular considerations were necessary to record the single unit neuron activity in deep structures of the brain in unanesthetized and unrestrained animals. However as in such past experiment, the holder of electrodes and micromanipulater system had been fixed on the cranial bone, so that the animals were under suffering unusual heavy weight on the head to maintain physiological posture and the recording of unit activity had been tend to limit in a definite neuron.<BR>To avoid these disadvantages, the special holder of synthetic resin with light weight was designed and the micro-insertion of electrodes was performed by means of oil pressing system which was situated in the out of animals. Furthermore, the position of holding electrode could be changed in some extent in this holder, so that it was possible to record the neuron activities in several parts of the brain stem in same animal and to record them for long time by selection of stable one from several neuron activities.<BR>Whenever the wanted unit activity was obtained, it was possible in this system to fix the electrode with dental cement for histlogical examination without disturbing another recordings.<BR>Among of several kinds of micro-electrodes, tungsten electrode was the most suitable for this system.<BR>As histological examinations, the frozen section method was best for comfirmation of the tip position of the electrode.

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