IMMUNOLOGIC STUDIES OF ERYTHROPOIETIN

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  • Erythropoietinの免疫学的研究
  • Erythropoietin ノ メンエキガクテキ ケンキュウ

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Immunologic studies were performed on the erytheropoietin with the antierythropoietin-sera (anti-E sera) obtained from rabbits immunized by erythropoietic active human anemic urine and phenylhydrazine-treated rabbits sera.<BR>Results were as follows :<BR>1) No marked changes of hemoglobin concentration, hematocrit, erythrocyte count and reticulocyte count were noted in the rabbits during the course of immunization for the production of anti-E sera.<BR>2) The titer of antierythropoietic activity of obtained sera had no specific relation with .circulating blood counts of immunized rabbits.<BR>3) Different level of antierythropoietic titer was obtained even immunized with same .antigenic material of same amount.<BR>4) Antisera obtained from rabbits immunized by human anemic urinary erythropoietin neutralized not only human anemic urine but phenylhydrazine-treated rabbits serum employing the bioassay method and immunological precipitation reaction.<BR>5) Different result of positive rate of erythropoietic activity in cord plasma was obtained as comparing two methods-bioassay and immunological precipitation reaction.<BR>6) Erythropoietic activity of anabolic hormone (s) was not neutralized by anti-E sera.

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