PARTICIPATION OF INTESTINAL DOPAMINE RECEPTOR IN CONSTIPATION OF PARKINSON'S DISEASE MODEL RAT

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  • Parkinson病モデルラットの便秘における腸管内ドパミン受容体の関与
  • Parkinsonビョウ モデル ラット ノ ベンピ ニ オケル チョウ カンナイ ドパミン ジュヨウタイ ノ カンヨ

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Parkinson's disease PD a progressive neurodegenerative disorder characterized by extrapyramidal symptoms, is often associated with non-motor symptoms. Constipation appears most frequently in the non-motor symptoms, and it is generally considered to be caused by autonomic disturbance. On the other hand, changes of enteric dopaminergic systems are also reported. It was reported that dopamine inhibits the intestine motility, and from among dopamine receptors, dopamine receptor D2 D2R in particular participates. The role of D2R in the constipation of PD is not clear. Therefore, the present study was conducted to clarify the mechanism of constipation in PD, especially the participation of D2R using the PD model rat. PD model rat was established by unilateral injection of 6-hydroxydopamine 6-OHDA into the medial forebrain bundle. Rats were bred in the metabolic cage and the amount of feces was measured for 28 days. The amount of feces of PD rats was significantly less than that of control rats. Thereafter, PD rat's colon was removed and the motility was recorded in Krebs solution. When apomorphine, that is the dopamine receptor agonist, was added to the Krebs solution, the movement was significantly inhibited compared with that of control rats. Moreover, D2R and tyrosine-hydroxylase TH were examined using immunohistofluorescent methods. The number of D2Rs in the colon was significantly increased in the PD group. There was no significant difference in TH-positive neurons dopaminergic neurons. These results suggested that the intestinal tract, at least in the colon, of the PD rat became more sensitive to dopamine due to the increase of the number of D2Rs, and had an influence in the movement of the intestinal tract.

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