内毒素血症と血液凝固系 Ｉ ひ細胞のｐｒｃｏａｇｕｌａｎｔ活性とその性状
書誌事項
- タイトル別名
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- Endotoxemia and blood coagulation
- 1. 脾細胞の procoagulant 活性とその性状
抄録
1. Increase in procoagulant activity (P-activity) of spleen cells from mouse given 50μg of S. typhimurium endotoxin (post-LPS-cells) was demonstrated as early as 4hrs after endotoxin. Time course of the procoagulant generation in the cells was the same as that of bone marrow cells.<br>2. P-activity in the post-LPS-cells was higher than that in normal-cells of control, however, no cytotoxic change was observed in the post-LPS-cells.<br>3. P-activity was destroyed by heating at 56°C for 10min.<br>4. P-activity of splenic mononuclear cells was higher than that of unfractionated whole nucleated cells.<br>5. After sonic disruption, splenic cellular fractions were separated by differential centrifugation. Most of the P-activity was found in the 14, 500×g sediment of the sonic lysate.<br>6. The procoagulant of the spleen cells (14, 500×g sediment or whole nucleated cells) could activate either intrinsic or extrinsic pathway, however, endotoxin could only enhance the activation of extrinsic pathway.<br>7. Parallelism between the dose response regression lines for post-LPS-cells or normalcells and mouse brain thromboplastin was recognized.<br>These results suggest that the P-activity is present in the cell membranes and the activity is enhanced as a result of the interaction of the cells with endotoxin in vivo. The procoagulant of post-LPS-cells is considered to be similar or identical to tissue thromboplastin. The participation of P-activity of splenic mononuclear cells would also be suggested in endotoxin-induced DIC.
収録刊行物
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- 血液と脈管
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血液と脈管 13 (2), 233-236, 1982
一般社団法人 日本血栓止血学会
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詳細情報 詳細情報について
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- CRID
- 1390001204858400512
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- NII論文ID
- 130004260751
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- ISSN
- 18842372
- 03869717
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可