A STUDY ON FERROCYANIDE-REDUCED OSMIUM TETROXIDE AS A STAIN AND CYTOCHEMICAL AGENT

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  • HOSHINO YOSHINOBU
    Department of Research Oncology and Cell Biology, Sinai Hospital of Baltimore Inc. Department of Surgery, The Johns Hopkins University School of Medicine Present address: Department of Urology, Fuchu Metropolitan Hospital
  • SHANNON Jr. W. ALLEN
    Department of Research Oncology and Cell Biology, Sinai Hospital of Baltimore Inc. Department of Surgery, The Johns Hopkins University School of Medicine Present address: Research Morphology and Cytochemistry Unit, General Medical Research, V. A. Hospital
  • SELIGMAN ARNOLD M.
    Department of Research Oncology and Cell Biology, Sinai Hospital of Baltimore Inc. Department of Surgery, The Johns Hopkins University School of Medicine Research Oncology and Cell Biology

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Ultrastructural and limited ultracytochemical studies were explored with potassium ferrocyanide-reduced osmium tetroxide (POCC). The use of 2% osmium tetroxide solution following POCC treatment was found to improve the preparation of ultrathin sections by facilitating the infiltration of Epon into the treated tissues. Potassium ferrocyanide alone did not stain tissues. Treating of tissues with potassium ferrocyanide and osmium tetroxide separately was not effective indicating that reduced osmium staining could not be produced within the tissues.

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