Sites of Glycosylation Observed by Postembedding and Preembedding Lectin Staining .

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  • MURATA FUSAYOSHI
    Department of Anatomy, Faculty of Medicine, Kagoshima University
  • TSUYAMA SHINICHIRO
    Department of Anatomy, Faculty of Medicine, Kagoshima University
  • KANAE TAKAKO
    Department of Anatomy, Faculty of Medicine, Kagoshima University
  • IHIDA KAORI
    Department of Anatomy, Faculty of Medicine, Kagoshima University
  • YANG DOHG-HUA
    Department of Anatomy, Faculty of Medicine, Kagoshima University

説明

Sites of glycosylation were investigated using postembedding and preembedding lectin staining. Positive labeling in the luminal side of perinuclear spaces and endoplasmic reticulum was observed by N-acetylgalactosamine and mannose binding lectins. Cis lamellae of the Golgi apparatus were stained by N-acetylgalactosamine and mannose binding lectins. Medial to trans lamellae were mainly labeled by galactose binding lectin and transmost lamellae were labeled by sialic acid and fucose binding lectins. The present results elucidated the route of glycoconjugate synthesis in relation to cell organelle.

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