二価鉄誘導脂質過酸化反応に関する研究

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タイトル別名
  • Studies on ferrous ion-induced lipid peroxidation
  • 二価鉄誘導脂質過酸化反応に関する研究-2-二価鉄誘導脂質過酸化反応における誘導期(lag)の二価鉄の消長による解析
  • 2カ テツ ユウドウ シシツ カサンカ ハンノウ ニカンスルケンキュウ 2 2
  • 第二編 二価鉄誘導脂質過酸化反応における誘導期(lag)の二価鉄の消長による解析
  • Part II Analysis of the induction period (lag) of ferrous ion-induced lipid peroxidation

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The author measured simultaneously Fe (II)-induced peroxidation of lipid extracted from rat liver mitochondria by the TBA method and the concentration of Fe (II) added to the medium quantitatively using Nitoroso-PSAP reagent.<br>1) An induction period (lag) at the early phase of the reaction of Fe (II)-induced lipid peroxidation was observed as well as in mitochondria.<br>2) After most of the Fe (II) concentration had decreased, the TBA-value, after lag-time, increased sharply.<br>3) Phosphate added to the medium shortened the lag-time. The lag-time in the presence of phosphate varied with the concentration and pH. However, in each case, there was a correlation between the decrement of Fe (II) concentration and the length of lag time as described above.<br>4) By adding EDTA for various reaction times, Fe was chelated immediately, then the TBA value did not increase.<br>5) The results suggest: iron interacted with the chain reaction of lipid peroxidation, mainly as Fe (II) and/or partially Fe (III); Fe (II) maintains the lag as a inhibitory action to the resolution into malondialdehyde(MDA) from lipid peroxide; Fe (III) was required for the formation of MDA.<br>6) Using these results, the author discussed the mechanisms of Fe (II)-induced lipid peroxidation.

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