Studies on ferrous ion-induced lipid peroxidation

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  • HASHIMOTO Keizi
    Department of Radiation Medicine, Okayama University Medical School

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Other Title
  • 二価鉄誘導脂質過酸化反応に関する研究
  • 二価鉄誘導脂質過酸化反応に関する研究-1-二価鉄誘導脂質過酸化反応液中の二価鉄の定量
  • 2カ テツ ユウドウ シシツ カサンカ ハンノウ ニカンスルケンキュウ 1 2
  • 第一編 二価鉄誘導脂質過酸化反応溶液中の二価鉄の定量
  • Part I Quantitative analysis of ferrous ion in a system of ferrous ion-induced lipid peroxidation

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Abstract

The 2-nitroso-(N-propyl-N-sulfopropylamino)phenol(Nitroso-PSAP), one of the watersoluble derivatives of nitrosoaminophenol, is a very sensitive reagent for the spectrophotometric determination of Fe (II). Using Nitroso-PSAP reagent, the author investigated the conditions for determining Fe (II) quantitatively and selectively the system of Fe (II)-induced lipid peroxidation, and obtained the following results.<br>1) Fe (II) could be measured quantitatively without reductant. The Fe (II) complex had a molar absorptivity of 4.5×104 dm3 mol-1 cm-1 at 756 nm.<br>2) Fe (II) could be measured quantitatively in the TCA solution removed from the reaction medium for lipid peroxidation. The Fe (II) complex had a molar absorptivity of 3.14×104 dm3 mol-1 at 756 nm.<br>3) Fe (II) could be measured quantitatively and selectivily under conditions involving a mixture of Fe (II).

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