Evaluation of Leucocyte Adherence Inhibition as an Immunodiagnostic Test for Pancreatic Carcinoma and Hepatocellular Carcinoma

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  • KONDO Hajime
    The Third Department of Internal Medicine, Kyorin University School of Medicine

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  • 膵癌および肝細胞癌における白血球粘着阻止試験の検討
  • スイガン オヨビ カン サイボウ ガン ニ オケル ハッケッキュウ ネンチャク

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Abstract

Leucocyte adherence inhibition (LAI) technique using nylon fiber column was applied to the diagnosis of pancreatic carcinoma and hepatocellular carcinoma. Tumor extracts (antigens) were prepared by homogenizing tumor tissue and stored at -20℃. Leucocyte-rich cell suspension (LRS) was prepared from heparinized venous blood. Nylon fiber weighing 65 mg was packed into 1 ml syringes having diameter of 5 mm. The length of packed column was adjusted to 17 mm. For the determination of adherence mixtures of LRS and tumor extracts, or PBS as control, were incubated for 30 min at 37℃. Then 1 ml of aliquots of the mixtures were added to the tops of syringes and allowed to filter through the columns over 5 to 10 minutes. All assays were done in triplicate. The numbers of the granulocytes in the original mixtures and in the effluents were counted, and the percentage of granulocytes adhered wasc alculated. The ratio of % adherence incubated with antigen to that of control sample was defined as LAI index. Mean LAI index of 20 patients with carcinoma of the pancreas was 0.73±0.19, which was significantly less than 0.93±0.08 of normal subjects (p<0.01). When the indices were less than 0.77 (mean LAI index of control group-2 SD), LAI tests were difind as positive. Thirteen of 20 cases (65.0%) showed positive results. False positive results were seen in 3 cases (carcinoma of the duodenum, renal cell carcinoma and pseudocyst of pancreas). Mean LAI index of 20 cases with hepatcellular carcinoma was 0.58±0.23, which was significantly less than 0.79±0.11 of 11 normal control cases (p<0.01). Positive results, indices less than 0.57, were obtained in 11 of 20 cases with hepatocellular carcinoma. No false positive case was seen. The LAI test is a reliable technique for the diagnosis of pancreatic carcinoma and hepatocellular carcinoma.

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