The Expression Level of the Exocytosis-related Protein during Recovery of the Transected Sciatic Nerve

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  • KUDO Fumitaka
    Department of Orthopedic Surgery, Kyorin University School of Medicine

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  • ラット坐骨神経切断後の修復過程における開口放出関連蛋白質の変動について
  • ラット ザコツ シンケイ セツダン ゴ ノ シュウフク カテイ ニ オケル カイコウ ホウシュツ カンレン タンパクシツ ノ ヘンドウ ニ ツイテ

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Abstract

SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, which play a central role in vesicle and plasma membrane transport processes, are localized at the growth cone and are considered to have an important role in neurite formation and regeneration. In order to evaluate chronological changes of protein expression in the rat spinal cord after transection of the rat sciatic nerve, we investigated the changes in the amount of expressed SNARE proteins such as HPC-1/syntaxin1A, SNAP25, and synaptotagmin-1 during peripheral nerve regeneration. Western blotting experiments showed that SNARE protein expression in the lumbar spinal cord decreased significantly 30 minutes after transection, while no change in the expression of this protein was observed in the thoracic spinal cord. Immunohistochemical studies showed that HPC-1/syntaxin 1A protein expression on the transected side of the lumbar spinal cord 30 minutes after transection was significantly lower than that before transection. SNAP25 protein expression was slightly but not significantly lower. c-Fos expression increased significantly in the ventral and dorsal horns 30 minutes after transection, and gradually recovered in three days. Taken that changes in the expression of SNARE proteins are thought to cause the sprouting and extension of axons, the results of the present study suggest that nerve regeneration begins quite soon after transection of the sciatic nerve.

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