Collaborative study on rat sperm motion analysis using Cellsoft Series 4000 semen analyzer.
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- BAN Yoshiki
- Safety Assessment, Banyu Pharmaceutical Co., Ltd.
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- NAYA Masato
- Toxicological Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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- NISHIMURA Tatsuya
- Fukui Institute for Safety Research, Ono Pharmaceutical Co., Ltd.
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- KANETO Masako
- Developmental Research Laboratories, Shionogi & Co., Ltd.
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- KISHI Kurajiro
- Developmental Research Laboratories, Shionogi & Co., Ltd.
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- INOUE Tadahiro
- Pharmaceutical Research Laboratories, Taisho Pharmaceutical Co., Ltd.
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- YOSHIZAKI Hiroshi
- Drug Safety Research Labs, Hikari Branch, Takeda Chemical Industries, Ltd.
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- OOSHIMA Yojiro
- Drug Safety Research Labs, Takeda Chemical Industries, Ltd.
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Abstract
A collaborative study was conducted to determine useful and sensitive rat sperm motion parameters in a CellSoft Series 4000 semen analyzer to detect the effects of compounds on sperm motion. The effects on the sperm motion parameters were investigated using α-chlorohydrin, boric acid, ethinylestradiol, ethyl methanesulfonate, nitrazepam, nitrobenzene, ornidazole, sulfasalazine or valproic acid which are well known to induce reproductive or testicular toxicities. All compounds used in this study decreased percentage of motile sperm (% motile). Curvilinear velocity (VCL), maximum and mean amplitude of lateral head displacement (ALH max and ALH mean) were decreased by treatment with all compounds except for valproic acid. Treatment with α-chlorohydrin, ornidazole or sulfasalazine under mid-dosage regimens decreased only these parameters. Beat cross frequency (BCF) was increased by treatment with sulfasalazine. There were some treatments which caused either decreased or increased changes irrespective of dosage regimen in linearity, average radius, percentage of circular-swimming sperm out of motile sperm (circular/motile) and percentage of circular-swimming sperm out of all sperm (circular/all). Based on these results, we concluded that % motile, VCL, ALH max and ALH mean are considered useful and sensitive parameters for evaluating the effects of compounds on sperm motion. A parameter of BCF can be useful to detect the effects of specific compounds on sperm motion. Linearity, average radius, circular/motile and circular/all are not considered useful or sensitive indicators to detect the effects of compounds on sperm motion.
Journal
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 26 (1), 9-24, 2001
The Japanese Society of Toxicology
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Details 詳細情報について
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- CRID
- 1390001204900911488
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- NII Article ID
- 110001806887
- 30013780584
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- NII Book ID
- AN00002808
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- DOI
- 10.2131/jts.26.9
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- COI
- 1:STN:280:DC%2BD3MzlsVeltA%3D%3D
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- ISSN
- 18803989
- 03881350
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- NDL BIB ID
- 5676828
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- PubMed
- 11255794
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed