Induction of unscheduled DNA synthesis in hairless mouse epidermis by 8-methoxypsoralen plus ultraviolet A(PUVA).
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- MORI Masaaki
- Life Science Research Center, Shiseido Co., Ltd.
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- KOBAYASHI Hiroshi
- Life Science Research Center, Shiseido Co., Ltd.
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- KATSUMURA Yoshio
- Life Science Research Center, Shiseido Co., Ltd.
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- FURIHATA Chie
- College of Science and Engineering, Aoyama-Gakuin University
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抄録
Induction of unscheduled DNA synthesis (UDS) by 8-methoxypsoralen (8-MOP) plus ultraviolet A (UV-A) (PUVA) was investigated in the epidermis of female hairless mice by means of an in vivo-in vitro assay using a liquid scintillation counting method. Groups of three to five 8-week-old female hairless mice had 8-MOP applied once onto two areas of the back after stripping of the stratum corneum with adhesive tape to enhance skin penetration, and were irradiated with UV-A. Skin samples were taken and cultured in a medium containing [3H]thymidine with or without hydroxyurea (HU) for 2 hr. DNA of the epidermis was extracted, and the incorporation of [3H]thymidine into DNA and the DNA content were determined with a liquid scintillation counter and a fluorescence spectrophotometer, respectively. Induction of UDS was judged in terms of the UDS index [(the ratio of DNA synthesis in the presence of HU to that in its absence)×100]. In a time-course study, the UDS index was increased at 1,2 and 24 hr after 1×105 J/m2 UV-A irradiation with 0.001% 8-MOP, reaching the maximum level at 24 hr. In a dose-response study, it was significantly increased at the dose of 1×105 J/m2 of UV-A at 24 hr with 0.001% 8-MOP, but showed no significant change at the doses of 0.5×105, 2×105 and 4×105 J/m2. In a further study on the effect of varying the dose of 8-MOP, the UDS index was significantly increased at 0.001 and 0.002% 8-MOP at 24 hr after 1×105 J/m2 UV-A irradiation, reaching the maximum level with 0.002% 8-MOP. The increase of the UDS index in these studies was less than 3-fold. These results show that PUVA causes a small induction of UDS, which might be due to slow DNA excision repair over a long period.
収録刊行物
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 26 (1), 1-8, 2001
一般社団法人 日本毒性学会
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詳細情報 詳細情報について
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- CRID
- 1390001204900913408
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- NII論文ID
- 110001806886
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- NII書誌ID
- AN00002808
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- DOI
- 10.2131/jts.26.1
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- COI
- 1:CAS:528:DC%2BD3MXjtlWksLc%3D
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- ISSN
- 18803989
- 03881350
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- NDL書誌ID
- 5676813
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可