<b>ENZYME IMMUNOASSAY FOR FLAVOENZYMES D-AMINO ACID OXIDASE AND QUASI D-AMINO ACID OXIDASE FROM HOG </b><b>KIDNEY </b>
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- KATAGIRI MASANAO
- Department of Biochemistry, Osaka University Medical School
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- TOJO HIROMASA
- Department of Biochemistry, Osaka University Medical School
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- HORIIKE KIHACHIRO
- Department of Biochemistry, Shiga University of Medical Science
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- KASUGA NAOKI
- Laboratory of Immuno Technology, Otsuka Pharmaceutical Co., Ltd.
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- IMAGAWA KENICHI
- Laboratory of Immuno Technology, Otsuka Pharmaceutical Co., Ltd.
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- YAMANO TOSHIO
- Department of Biochemistry, Osaka University Medical School
説明
<p>We have developed an enzyme immunoassay (EIA) system (‘sandwich method’) for a flavoenzyme, D-amino acid oxidase (DAO), employing polystyrene beads as a solid-phase, and Fab’-horseradish peroxidase conjugate. This assay system is accurate and precise in the concentration range of 1.0—100 ng/ml, and up to 26 h at 4°C. At 37°C, the measurable DAO concentration range is considerably decreased. DAO and quasi D-amino acid oxidase (Quasi-DAO) completely crossreacted with the anti-DAO antibody both in the double immunodiffusion test, and in the assay with the EIA system. The system is therefore useful for measuring the total content of DAO and Quasi-DAO. The DAO content in crude samples such as the kidney homogenate and serum can be accurately measured. In hog serum, DAO was not detectable; in hog kidney cortex extract, DAO was estimated to be 2.2 ± 0.6 μg/mg protein. DAO was irreversibly polymerized on iodination by the chloramine T method, and the nonspecific counts were very high, which caused our inability to use a radioimmunoassay in the determination of DAO.</p>
収録刊行物
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- Biomedical Research
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Biomedical Research 5 (2), 125-134, 1984
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詳細情報 詳細情報について
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- CRID
- 1390001204901718400
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- NII論文ID
- 130005975393
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- ISSN
- 1880313X
- 03886107
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
