A TIME-COURSE CHARACTERIZATION OF MALE REPRODUCTIVE TOXICITY IN RATS TREATED WITH METHYL METHANESULPHONATE (MMS)
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- KURIYAMA Kazuya
- Toxicology research laboratories, Kissei Pharmaceutical Co., Ltd.
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- YOKOI Ryohei
- Toxicology research laboratories, Kissei Pharmaceutical Co., Ltd.
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- KOBAYASHI Kazuo
- Toxicology research laboratories, Kissei Pharmaceutical Co., Ltd.
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- SUDA Satoshi
- Toxicology research laboratories, Kissei Pharmaceutical Co., Ltd.
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- HAYASHI Morimichi
- Toxicology research laboratories, Kissei Pharmaceutical Co., Ltd.
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- OZAWA Shigenari
- Toxicology research laboratories, Kissei Pharmaceutical Co., Ltd.
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- KURODA Junji
- Toxicology research laboratories, Kissei Pharmaceutical Co., Ltd.
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- TSUJII Hirotada
- Faculty of Agriculture, Shinshu University
Bibliographic Information
- Other Title
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- time course characterization of male reproductive toxicity in rats treated with methyl methanesulphonate MMS
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Abstract
Methyl methanesulphonate (MMS), a potent alkylating agent and testicular toxicant, was orally administered to rats for 5 days at doses of 20, 30, and 40 mg/kg. During the recovery period of 5 weeks, males were evaluated for multiple endpoints such as organ weights, fertility, and sperm parameters. The 5-week recovery periods are designated as follows: Day 1 (1 day after final treatment); Week 1, Week 2, Week 3, Week 4, and Week 5 (first, second, third, fourth, and fifth week after final treatment). A clear time-course of dominant lethals was observed. The peak severities of the dominant lethals were observed in Week 2. It was judged that the most sensitive cellular targets for the dominant lethals are late spermatids. Sperm examination revealed a clear time-course and dose-dependent changes in the frequency of sperm morphological abnormalities. The peak severities of the sperm morphological alterations in cauda epididymis were observed in Week 4. Sensitive cellular stages for the induction of sperm morphological abnormalities were judged to be late spermatocytes and early spermatids. The most frequently observed type of morphologically abnormal spermatozoa was tailless sperm, followed by no-hook head sperm. Although the initial cause for both sperm morphological alterations and dominant lethals was suggested to be genetic insult to the germ cells, there were no obvious relationships observed between these two findings.<br>
Journal
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 30 (2), 91-102, 2005
The Japanese Society of Toxicology
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Details
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- CRID
- 1390001204902815616
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- NII Article ID
- 110001817039
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- NII Book ID
- AN00002808
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- ISSN
- 18803989
- 03881350
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- NDL BIB ID
- 7375930
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- PubMed
- 15928457
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed