Insulin Binding to Plasma Membranes in Ascites Hepatoma Cells

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  • Sasaki Takashi
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Nakayama Hidetaka
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Watanabe Takuji
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Ozaki Shiro
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Aoki Shin
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Oda Kazuaki
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Kurihara Yoshio
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Sato Mituo
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Nakagawa Shoichi
    The Second Department of Medicine, Hokkaido University School of Medicine
  • Makita Akira
    Biochemical Laboratory, Cancer Institute, Hokkaido University School of Medicine

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Other Title
  • 腹水肝癌細胞のインスリンレセプターに関する研究
  • フクスイ カンガン サイボウ ノ インスリン レセプター ニカンスルケンキュウ

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Abstract

The nature of insulin receptors in ascites hepatoma AH-66 were studied. The hepatoma plasma membranes were found to contain at least two types of insulin receptors with affinity constants of 9.0 × 109M-1 and 2.8 × 109M-1. Their affinity constants were almost equal to those in plasma membranes from normal rat liver. The capacity of the high affinity binding sites in AH-66 cells was larger by 50% as compared with that of normal liver. No differences were detected between AH-66 cells and normal livers in the inhibiting effect of concanavalin A on 125I-insulin binbing. Furthermore, neither glucagon, ACTH, TSH, nor HGH inhibited 125I-insulin binding to plasma membranes from AH-66 cells. Addition of 2mM N-ethylmaleimide to the assay system increased the affinity constant, binding capacity, and specific 125I-insulin binding.<BR>These results indicate that the plasma membrane of ascites hepatoma AH-66 has high and low affinity insulin receptors which are specific for insulin as that of the normal liver cell.

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